Unfused C2C12 Mouse Skeletal Muscle Cells Express Neurofilament 140K Protein.
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- Matsuda Ryoichi
- Department of Life Sciences, The University of Tokyo
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- Abe Masami
- Department of Life Sciences, The University of Tokyo Present address: Sapporo Institute, Sangi Co, Inc.
書誌事項
- タイトル別名
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- Unfused C2C12 Mouse Skeletal Muscle Cel
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Expression of neurofilament 140K protein in C2C12 mouse skeletal muscle cells was studied. Immunofluorescence and immunoblot analyses revealed that NF140K was expressed at the proliferative stage and was colocalized with the muscle-specific intermediate protein desmin. As muscle cell differentiation proceeded, the number of NF140K-positive cells decreased whereas the number of cells expressing muscle-specific marker proteins such as sarcomeric myosin heavy chain and troponin-T increased. Down-regulation of NF140K and upregulation of a myogenic regulatory gene, the myogenin gene, started simultaneously. In differentiated muscle cell cultures, unfused cells residing between myotubes remained NF140K-positive. NF140K and desmin doublepositive cells were also found in a primary culture of adult mouse skeletal muscle cells. The results suggest that NF140K may be a unique marker for uncommitted myoblasts.
収録刊行物
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- Cell Structure and Function
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Cell Structure and Function 22 (1), 117-121, 1997
日本細胞生物学会
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詳細情報 詳細情報について
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- CRID
- 1390282679672126208
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- NII論文ID
- 130003512993
- 10001916481
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- NII書誌ID
- AA0060007X
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- COI
- 1:CAS:528:DyaK2sXivVGntL8%3D
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- ISSN
- 13473700
- 03867196
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- NDL書誌ID
- 4188142
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- PubMed
- 9113397
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可