Establishment and Characterization of Human Peripheral Nerve Microvascular Endothelial Cell Lines: A New <i>in vitro</i> Blood-Nerve Barrier (BNB) Model
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- Abe Masaaki
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
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- Sano Yasuteru
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
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- Maeda Toshihiko
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
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- Shimizu Fumitaka
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
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- Kashiwamura Yoko
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
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- Haruki Hiroyo
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
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- Saito Kazuyuki
- Department of Neurology and Neurological Science, Tokyo Medical and Dental University Graduate School Department of Neurology and Neurological Science, Tokyo Medical and Dental University Graduate School
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- Tasaki Ayako
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
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- Kawai Motoharu
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
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- Terasaki Tetsuya
- Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University Department of Molecular Biopharmacy and Genetics, Graduate School of Pharmaceutical Sciences, Tohoku University
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- Kanda Takashi
- Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine Department of Neurology and Clinical Neuroscience, Yamaguchi University Graduate School of Medicine
Bibliographic Information
- Other Title
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- Establishment and Characterization of Human Peripheral Nerve Microvascular Endothelial Cell Lines : A New in vitro Blood-Nerve Barrier (BNB) Model
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Abstract
The blood-nerve barrier (BNB) is a highly specialized unit that maintains the microenvironments of the peripheral nervous system. Since the breakdown of the BNB has been considered a key step in autoimmune neuropathies such as Guillain-Barré syndrome and chronic inflammatory demyelinating polyraduculoneuropathy, it is important to understand the cellular properties of the peripheral nerve microvascular endothelial cells (PnMECs) which constitute the BNB. For this purpose, we established an immortalized cell line derived from human PnMECs. The human PnMECs were transduced with retroviral vectors encoding the temperature-sensitive SV40 large T antigen and human telomerase. This cell line, termed FH-BNB, showed a spindle fiber-shaped morphology, expression of von Willebrand factor and uptake of acetylated low density lipoprotein. These cells expressed tight junction proteins including occludin, claudin-5, ZO-1 and ZO-2 at the cell-cell boundaries. P-glycoprotein and GLUT-1 were also detected by a Western blot analysis and the cells exhibited the functional expression of p-glycoprotein. In addition, transendothelial electrical resistance experiments and paracellular permeabilities of sodium fluorescein and fluorescein isothiocyanate-labeled dextran of molecular weight 4 kDa across these cells demonstrated that FH-BNBs had functional tight junctions. These results indicated that FH-BNBs had highly specialized barrier properties and they might therefore be a useful tool to analyze the pathophysiology of various neuropathies.
Journal
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- Cell Structure and Function
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Cell Structure and Function 37 (2), 89-100, 2012
Japan Society for Cell Biology
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Details 詳細情報について
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- CRID
- 1390282679672516480
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- NII Article ID
- 130004137580
- 40019581994
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- NII Book ID
- AA0060007X
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- COI
- 1:STN:280:DC%2BC38npsV2jsg%3D%3D
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- ISSN
- 13473700
- 03867196
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- NDL BIB ID
- 024272253
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- PubMed
- 22672995
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed