Interaction of alpha-taxilin Localized on Intracellular Components with the Microtubule Cytoskeleton

DOI Web Site Web Site PubMed 被引用文献3件 参考文献44件
  • Horii Yukimi
    Department of Molecular and Cell Biology, Graduate School of Medicine, Dokkyo Medical University
  • Nogami Satoru
    Department of Molecular and Cell Biology, Graduate School of Medicine, Dokkyo Medical University
  • Kawano Yoji
    Department of Molecular and Cell Biology, Graduate School of Medicine, Dokkyo Medical University
  • Kaneko-Kawano Takako
    Department of Molecular and Cell Biology, Graduate School of Medicine, Dokkyo Medical University
  • Ohtomo Natsuko
    Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo
  • Tomiya Tomoaki
    Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo
  • Shirataki Hiromichi
    Department of Molecular and Cell Biology, Graduate School of Medicine, Dokkyo Medical University

書誌事項

タイトル別名
  • Interaction of α-taxilin Localized on Intracellular Components with the Microtubule Cytoskeleton

この論文をさがす

抄録

Intracellular vesicle traffic plays an essential role in the establishment and maintenance of organelle identity and biosynthetic transport. We have identified α-taxilin as a binding partner of the syntaxin family, which is involved in intracellular vesicle traffic. Recently, we have found that α-taxilin is over-expressed in malignant tissues including hepatocellular carcinoma and renal cell carcinoma. However, a precise role of α-taxilin in intracellular vesicle traffic and carcinogenesis remains unclear. Then, we first investigated here the intracellular distribution of α-taxilin in Hela cells. Immunofluorescence studies showed that α-taxilin distributes throughout the cytoplasm and exhibits a tubulo-vesicular pattern. Biochemical studies showed that α-taxilin is abundantly localized on intracellular components as a peripheral membrane protein. Moreover, we found that α-taxilin distributes in microtubule-dependent and syntaxin-independent manners, that α-taxilin directly binds to polymerized tubulin in vitro, and that N-ethylmaleimide but not brefeldin A affects the intracellular distribution of α-taxilin. These results indicate that α-taxilin is localized on intracellular components in a syntaxin-independent manner and that the α-taxilin-containing intracellular components are associated with the microtubule cytoskeleton and suggest that α-taxilin functions as a linker protein between the α-taxilin-containing intracellular components and the microtubule cytoskeleton.

収録刊行物

被引用文献 (3)*注記

もっと見る

参考文献 (44)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ