A Rapid and Simple Method Using the Detergent Triton X114 for Preparation of Outer Membrane Proteins from Bacteria Containing Mycolic Acids.

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  • Aizawa Tomoko
    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University
  • Iwabuchi Noriyuki
    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University
  • Kikuchi Aki
    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University
  • Urai Makoto
    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University
  • Anzai Hirosi
    Laboratory of Applied Biochemistry, Department of Agriculture, Junior College, Nihon University
  • Sunairi Michio
    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University
  • Nakajima Mutsuyasu
    Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University

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  • rapid and simple method using the detergent Triton X114 for preparation of outer membrane proteins from bacteria containing mycolic acids

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Description

A rapid and simple method using the detergent Triton X114 to prepare outer membrane proteins from whole cells of bacteria containing mycolic acids, α-alkyl, β-hydroxyl fatty acids is described. SDS-PAGE analysis and subsequent N-terminal amino acid sequencing revealed that the proteins prepared from whole cells by this method were almost identical to outer membrane proteins prepared by the conventional cell-disruption and differential-centrifugation method. Therefore, this method is useful to prepare outer membrane proteins from whole cells of Mycolata, bacteria containing mycolic acids.

Journal

  • Actinomycetologica

    Actinomycetologica 15 (1), 6-10, 2001

    The Society for Actinomycetes Japan

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