Estimation of Vitamin E Concentration in Swine Serum by Hemolysis Test with Hydrogen Peroxide

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  • 豚の過酸化水素溶血試験と血清中Vitamin E濃度との関係
  • ブタ ノ カサンカ スイソ ヨウケツ シケン ト ケッセイチュウ Vitami

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Abstract

Two methods of hemolysis test with hydrogen peroxide (one devised by Ikehata et al. and the other modified by the present authors) were evaluated to estimate the concentration of vitamin E (VE) in swine serum.<BR>In experiment 1, 25 blood samples were obtained from 9 gilts fed a VE-poor, VE-rich or ordinary diet for about 1-3 months. When estimated by the method of Ikehata et al., the hemolysis rate averaged 58%, ranging from 3 to 93%, in them. The serum concentration of VE as α-tocopherol in the same samples was determined by the method of Abe and Katsui with high speed liquid chromatography. It averaged 0.18mg/dl, ranging from 0.03 to 0.52 mg/dl. There was a highly significant negative correlation between the hemolysis rate (X) and the VE level (Y) in these samples (Y=-0.005X+ 0.464, r=-0.93, P<0.01).<BR>In experiment 2, 10 blood samples were collected in heparinized vacuum tubes from 8 gilts fed diets at different VE levels for 5-14 months. In them, the hemolysis rate by the method of Ikehata et al.(X) and that by the authors' modified method (Y) almost coincided with each other. A highly significant correlation was observed between them (Y=0.995X+0.98, r=0.995, P<0.01).<BR>In conclusion, the modified method seemed to be simpler and more effective than the original one for the screening test of VE deficiency in swine.

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