ＰＣＲ法によるブタのＳＲＹ保存領域の検出

DOI

書誌事項

タイトル別名
  • Detection of SRY (sex-determining region Y) Conserved Region in Pig Leukosytes using Polimerase Chain Reaction.

この論文をさがす

抄録

In 1900, the sequence of sex-determining region of Y (SRY) gene was reported. This gene considered as Y-specific gene over the wide range of mammalian species. In this study, as a first step of sexing of pig embryo, we attempted to explore the method to detect the SRY conserved region in pig leucocyte using polymerase chain reaction (PCR). The primer was prepared on the SRY conserved sequences of human, mouse and rabbit; sense primer, 5′-GTCAAGCGACCCATGAACGC-3′ (20mer), antisence primer, 5′-CTGTGCCTCCTGGAAGAATGGC-3′ (22mer). PCR was performed for 30 cycles of amplification (denaturation at 94°C for 1.5min, annealing at 60°C for 2.0min, and extension at 72°C for 1.5min.). After amplification, target DNA fragment was detected only in male DNA sample. Furthermore, the increase of amplifing frequency from 30 to 50 cycles was able to detect the objective band even in the sample containing 5pg DNA.

収録刊行物

キーワード

詳細情報 詳細情報について

  • CRID
    1390282679684243328
  • NII論文ID
    130004388667
  • NII書誌ID
    AN10202971
  • DOI
    10.5938/youton.30.125
  • ISSN
    1881655X
    0913882X
  • 本文言語コード
    ja
  • データソース種別
    • JaLC
    • Crossref
    • CiNii Articles
  • 抄録ライセンスフラグ
    使用不可

問題の指摘

ページトップへ