Detection by Means of the Latex-agglutination Test of the Canine Parvovirus in Inoculated and Naturally Infected Dogs

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  • 生ワクチン接種犬および野外感染犬におけるラテックス凝集反応による犬パルボウイルスの検出
  • ナマ ワクチン セッシュケン オヨビ ヤガイ カンセンケン ニ オケル ラテックス ギョウシュウ ハンノウ ニ ヨル イヌ パルボウイルス ノ ケンシュツ

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Abstract

The canine parvovirus (CPV) excreted in the feces of dogs inoculated with live CPV vaccine and othernaturally infected dogs was examined by means of the Latex-agglutination test (LA test). The virus was isolated from the feces of the inoculated dogs, though none of them manifested clinical signs. In2 inoculated dogs, at 6 weeks of age, the LA test revealed no CPV-specific antigen; and the ELISA test detected low virus titers of from 2 to 5 units. In 2 of 4 dogs inoculated at 15 days of age, the LA test showed weak agglutination in undiluted feces solution. In the ELISA test, 4 dogs excreted virus titers of from 3 to 100 units. CPV was isolated from 7 animals manifesting severe clinical signs. The HA test on feces from these animals showed virus titers of from 1: 512 to 1: 4, 096; the LA test showed titers of from 1: 2 to 1: 512. These results suggest that, in small animal practice theLA test is useful in discriminating rapidly between CPV natural infection and CPV infection resulting from vaccination.

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