Evaluation of Isolation and Identification Methods for <I>Arcobacter butzleri</I> and <I>Arcobacter cryaerophilus</I> and the Prevalence of These Species in Chicken Meat

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  • MARUYAMA Soichi
    Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University
  • HOSHI Minori
    Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University
  • MORITA Yukio
    群馬県衛生環境研究所
  • KABEYA Hidenori
    Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University
  • KATSUBE Yasuji
    Laboratory of Veterinary Public Health, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University

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Other Title
  • <I>Arcobacter butzleri</I>と<I>A. cryaerophilus</I>の分離・同定法の検討ならびに鶏肉の汚染状況
  • Arcobacter butzleriとA.cryaerophilusの分離・同定法の検討ならびに鶏肉の汚染状況
  • Arcobacter butzleri ト A cryaerophilus ノ ブンリ ドウテイホウ ノ ケントウ ナラビニ ケイニク ノ オセン ジョウキョウ
  • Evaluation of Isolation and Identification Methods for Arcobacter butzleri and Arcobacter cryaerophilus and the Prevalence of These Species in Chicken Meat

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Abstract

Optimum enrichment broth, selective isolation media, and their culture conditions were examined for the purposes of isolating Arcobacter butzleri and Arcobacter cryaerophilus. Optimum enrichment-culture conditions for the Arcobacter species were obtained by using Arcobacter Selective Broth at 25°C for 2 to 3 days under aerobic conditions. An aerobic culture using modified CIN agar and either Skirrow blood agar or Preston blood agar at 25°C for 2 to 3 days was suitable for isolating both Arcobacter species. Results of tests for catalase, nitrate reduction, 0.04% TTC resistance, and nalidixic-acid resistance and polymerase chain reactions differentiated A. butzleri, A. cryaerophilus, and thermophilic Campylobacters. In addition, the prevalence of Arcobacter species was investigated in 80 samples of retail chicken meat and 20 chicken livers. Twenty percent of the 180 samples were contaminated with Arcobacter species.A. butzleri was isolated from 11 samples, A.cryaerophilus from 6 samples, and both A. butzleri and A. cryaerophilus from 3 samples.

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