Assessment of Protein G in Serodiagnosis of Zoo Animals and Development of an Enzyme-Linked Immunosorbent Assay for Asian Elephant

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  • SHIMOSAWA Kentaro
    Department of Veterinary Public Health, Faculty of Agriculture, University of Miyazaki
  • MISAWA Naoaki
    Department of Veterinary Public Health, Faculty of Agriculture, University of Miyazaki

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Other Title
  • 動物園動物の血清診断におけるプロテインGの有用性とアジァゾウにおけるELISA法の確立
  • 動物園動物の血清診断におけるプロテインGの有用性とアジアゾウにおけるELISA法の確立
  • ドウブツエン ドウブツ ノ ケッセイ シンダン ニ オケル プロテイン G ノ ユウヨウセイ ト アジアゾウ ニ オケル ELISAホウ ノ カクリツ

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Abstract

Enzyme-Linked Immunosorbent Assay (ELISA), a fast and cost-effective serodiagnosis, is a helpful screeningmethod in the diagnosis of infectious diseases. However, it remains unclear whether ELISA is suitable forthe diagnosis of zoo animals. This study examined the reactivity of Protein G, an IgG-binding protein, to serafrom zoo animals. Results showed that most sera examined bound strongly to Protein G, while sera from marsupialanimals, Panthera and Asian elephant showed weak reactions. These findings suggest that Protein Gdoes not bind IgG uniformly in zoo animals. IgG was purified from Asian elephant serum, andused to obtainantiserum by immunizing a rabbit. This made it possible to develop an ELISA system for effectively detectingIgG in an Asian elephant.

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