An Improved Method for Isolation of Pathogenic Bacteria from Water

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Other Title
  • 水中の病原性細菌の定性的検出法
  • 水中の病原性細菌の定性的検出法--塩化第2鉄による沈殿法の改良法の開発
  • スイチュウ ノ ビョウゲンセイ サイキン ノ テイセイテキ ケンシュツホウ エ
  • Modified Precipitation Method with Diatomaceous Earth
  • 塩化第二鉄による沈殿法の改良法の開発

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Abstract

An improved method was developed from the conventional precipitation method for isolation of pathogenic bacteria from water.<BR>Various kinds of bacteria grown in culture media were suspended in sterilized water. Ferric chloride and diatomaceous earth were added to the resulting suspension to a final concentration of 0.02 % and 0.1 %, respectively. The suspensions were adjusted to be neutral or slightly alkaline with 1 N sodium hydroxide solution. They were allowed to make precipitates for 90 minutes. Then the supernatants were removed. It was demonstrated that the precipitates held a majority of the bacteria. Furthermore, the bacteria could be recovered from the precipitate even if only a few bacteria (less than 100 cfu/liter) were suspended in the original water. This method is applicable to various kinds of water, such as drinking water, surface water, and drainage, if an adequate amount of diatomaceous earth is added to the water.

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