生乳の色素還元試験法, とくにレサズリン試験紙中のレサズリン反応速度

大塚 義一

doi:10.12935/jvma1951.21.641

生乳の色素還元試験法, とくにレサズリン試験紙中のレサズリン反応速度

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大塚義一

雪印乳業株式会社東京市乳アイスクリーム事業部

書誌事項

タイトル別名

Dye Reduction Test of Fresh Milk with Special Reference to Reaction Velocity in Resazurin Test Paper
ナマニュウノシキソカンゲンシケンホウ , トクニレサズリンシケンシチュウノレサズリンハンノウソクド

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抄録

It was clarified that the reaction velocity at which resazurin (RZ) in RZ test paper changed to resorufin belonged to the firstorder reaction. When RZ test paper containing l0s S.P.C. per ml of fresh milk was cultured at 37°C, the velocity constant was 0.014. It was presumed from these results that the RZ reaction might have been in progress normally in the RZ test paper. Accordingly, this paper was known to be used for a practical test with success.<BR>The reaction velocity of RZ in fresh milk contained to the test paper was almost doubled when there was a rise in temperature by ten degrees centigrade. Incubation of RZ test paper at 18°C for 4.5 hours was to give the same result as that of conventional culture at 37°C for an hour theoretically.