Detection of Norovirus Genes in Imported Fresh Seafood

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  • 輸入生鮮魚介類からのノロウイルス遺伝子検出状況
  • 日本獣医公衆衛生学会会誌 輸入生鮮魚介類からのノロウイルス遺伝子検出状況
  • ニホン ジュウイ コウシュウ エイセイ ガッカイカイシ ユニュウ セイセン ギョカイルイ カラ ノ ノロウイルス イデンシ ケンシュツ ジョウキョウ

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Abstract

A combination of real-time PCR and reverse-transcription PCR was used to screen for the presence of noroviruses (NVs) in samples of imported seafood obtained from the C City central wholesale market between August 2001 and January 2003. NVs were detected in 13.0% of the samples tested. NV-contamination rates for each type of seafood was as follows: scallops 22.2%, hard clams 16.7%, black tiger shrimp 14.3%, and were detected ark shells 9.9%. Among NV-positive specimens, the G I genogroup only was detected in 28.6%, the Gil genogroup was detected only in 60.7%, and both the G I and the G II genogroups in 10.7%. These results clearly show that the NVs enter Japan via fresh imported seafood. Monitoring of viral contamination in such products is therefore essential.

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