Phylogenetic Analysis of Bluetongue Viruses Spread in Tochigi Prefecture, Japan

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  • 栃木県におけるブルータングウイルスの流行と検出遺伝子の分子系統樹解析
  • トチギケン ニ オケル ブルータングウイルス ノ リュウコウ ト ケンシュツ イデンシ ノ ブンシ ケイトウジュ カイセキ

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Abstract

In 2005, the bluetongue virus (BTV) was prevalent in cattle bred in Tochigi Prefecture, Japan. The antibodies to BTV were detected initially in September and were the earliest, covering a wide area since the first outbreak of BTV in that area in 1994. The three kinds of RNA segments of BTV detected between 2001 and 2005 were phylogenetically analyzed. By analysis of RNA segment 3 encoding of the inner capsid protein VP3, the Japanese BTVs including the Tochigi strains were classified into the same genogroup and closely related to some strains derived from Asia and Australia. Although the result of phylogenetic analysis of RNA segment 10 encoding non-structural protein NS3/3A was similar to that of RNA segment 3, the Tochigi strains were grouped into two distinct lineages. The analysis of RNA segment 2 encoding of the outer capsid protein VP2,the serotype-specific neutralizing antigen, revealed that the BTV serotype 21 has appeared repeatedly in Tochigi Prefecture. BTV might have been introduced to Japan from a neighboring country.

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