Nonspecific Reaction in ELISA Observed at a Farm that was Negative for Porcine Reproductive and Respiratory Syndrome Virus

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  • 豚繁殖・呼吸障害症候群ウイルス陰性農場にみられたELISAの非特異反応
  • ブタ ハンショク コキュウ ショウガイ ショウコウグン ウイルス インセイ ノウジョウ ニ ミラレタ ELISA ノ ヒトクイ ハンノウ

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Abstract

A farm that did not have porcine reproductive and respiratory syndrome (PRRS) virus had several pigs found to be antibody-positive in an ELISA (seropositive pigs). The positive rate was 5.4% among growing pigs and 0% among breeding sows and fattening pigs. Diagnostic tests on the five positive pigs ruled out PRRS. Serum of the seropositive pigs showed an ELISA S/P of 0.431 to 3.346, but had an IFA titer of less than 1 : 20, and were negative for nested PCR. It was concluded that all of the seropositive pigs had shown a nonspecific reaction. Investigating the possibility of infection by checking clinical symptoms, and conducting IFA and nested PCR with seropositive pigs appeared to be an effective procedure for verifying nonspecific reactions in an ELISA.

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