A Bovine Case Report of Mycobacteriosis

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  • 非定型抗酸菌による牛の1症例
  • ヒテイケイ コウサンキン ニ ヨル ウシ ノ 1 ショウレイ

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Four strains of acid-fast bacilli were isolated from a cow diagnosed as tuberculosis macroscopically and histologically at autopsy. In the primary isolation, small smooth colonies appeared about 20 days after cultivation and growth was stimulated by glycerol. Later, colonies were enlarged, but not pigmented.<BR>These isolates were weakly positive for catalase activity, but negative for niacin, nitrate reduction, urease, Tween 80 hydrolysis, and formamidase tests.<BR>Guinea pigs inoculated intraperitoneally with 0.2ml of 12-day-old culture in Dubos medium of these strains reacted more strongly to avian than mammalian PPD. At atuopsy, they has small lesions at the site of inoculation, in the peritoneal membrane, Liver, and spleen. The inoculated acid-fast bacilli were recovered from the lesions.<BR>No macroscopic lesions were observed in rabbits inoculated intravenously with 1.6×108bacilli, which were recovered from lung, spleen, and liver. There were no microscopic findings showing the presence of acid-fast bacilli. The isolates could be identified to beMycobacterium intracellulare.

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