Rapid Detection of<I>Theileria sergenti</I>and Other Protozoa by Fluorescence Microscopy with 4'6-Diamidino-2-Phenylindole (DAPI)

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  • 4'6-Diamidino-2-Phenylindole (DAPI) による原虫, とくに小型ピロプラズマの検出
  • 4′6-Diamidino-2-Phenylindole(DAPI)による原虫,とくに小型ピロプラズマの検出
  • 4 6 Diamidino 2 Phenylindole DAPI ニ ヨル
  • Rapid Detection ofTheileria sergentiand Other Protozoa by Fluorescence Microscopy with 4'6-Diamidino-2-Phenylindole (DAPI)

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Abstract

Diagnostic value of DAPI staining was discussed infections withTheileria sergentiand other protozoa. DAPI, capable of binding to double-stranded DNA, stained the nucleus of T. sergenti giving a strong fluorescence which figureed out the characteristic morphologica 1 profile of the nucleus of T. sergenti. This unique positive staining pattern made it easy to detect the parasite in the blood of infected animals.<BR>While the conventional Histopathological method, Giemsa staining, gave a high false negative and a false positive rate, a statistical study showed that this DAPI staining gave high reliability in the diagnosis of T.sergenti, resulting in 12.2% improvement.

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