Detection of Anti-<i>Brucella canis </i>Agglutination Antibodies by Microtiter and, Study the Effects of Hemolysis

DOI JASI Web Site Web Site 2 Citations 10 References

Bibliographic Information

Other Title
  • マイクロタイター法による抗ブルセラカニス凝集抗体の検出並びに，溶血による影響の検討
  • マイクロタイターホウ ニ ヨル コウブルセラカニス ギョウシュウ コウタイ ノ ケンシュツ ナラビニ,ヨウ チ ニ ヨル エイキョウ ノ ケントウ

Search this article

Abstract

In the present study, we compared the activities of anti-Brucella canis agglutination antibodies obtained by the traditionally used tube agglutination (TA) method and the microtiter method (MA). In addition, we studied the relationships between hemoglobin (HGB) concentration, considered to be a factor in nonspecific agglutination, and the nonspecific agglutination activity by analyzing dog plasma samples. The results from MA and TA were in complete accordance in all dog plasma samples obtained from 72 seronegative and 46 seropositive dogs. A high correlation coefficient (R ＝0.938) was shown between agglutination titers (log) tested by both methods. To study the effect of hemolysis, we obtained whole blood samples from nine specific-pathogen free(SPF) beagles and then intentionally hemolyzed the samples. The supernatants were diluted to various concentrations of HGB and the agglutination titers were determined. The results obtained showed a high correlation coefficient (R ＝0.912) between the HGB concentrations and agglutination titers. The lowest HGB concentration was 0.5 g/dl among diluents with 1 : 160 or higher antibody titers (positive criterion). These observations suggested that HGB was an important factor in nonspecific agglutination, and therefore, that predetermination of HGB concentration would be helpful in testing hemolyzed plasma.

Journal

Citations (2)*help

See more

References(10)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top