High Density Culture of EPA-Rich <I>Nannochloropsis</I> Using the Treated Wastewater from Rotifer Culture and Its Subsequent Application to Continuous Culture of Rotifer <I>Brachionus plicatilis</I>

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  • Kobayashi Takayuki
    Central Research Laboratory, Ebarajitsugyo Co., Ltd. Department of Marine Biosciences, Tokyo University of Marine Science and Technology
  • Nagase Toshiya
    Central Research Laboratory, Ebarajitsugyo Co., Ltd.
  • Katayama Yasuto
    Central Research Laboratory, Ebarajitsugyo Co., Ltd.
  • Hino Akinori
    Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • Takeuchi Toshio
    Department of Marine Biosciences, Tokyo University of Marine Science and Technology

Bibliographic Information

Other Title
  • ワムシ排処理水を利用したEPA豊富な高密度ナンノクロロプシス培養とそのワムシ連続培養への応用
  • High density culture of EPA Rich Nannochloropsis using the treated wastewater from rotifer culture and its subsequent application to continuous culture of rotifer Brachionus plicatilis

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Abstract

A high density culture of Nannochloropsis oculata rich in eicosapentaenoic acid (EPA) using the treated wastewater from rotifer culture and its application to the continuous culture of rotifer Brachionus plicatilis were examined. First, a continuous rotifer culture provided with freshwater Chlorella was established, and the rotifer wastewater was treated and filtered using their unit systems. 50% of the Nannochloropsis culture water was replaced with the treated wastewater. For the Nannochloropsis culture, three plain water tanks were linked and set up outdoors. By feeding the harvested raw Nannochloropsis to rotifer, a continuous rotifer culture was conducted. In the wastewater treatment for reuse of the rotifer culture water, the total nitrogen, total phosphorus, chemical oxygen demand, and suspended solid concentrations in the inflow water significantly decreased (P<0.05) and thus reducing the total pollution loading of rotifer culture. The cultured Nannochloropsis could be harvested at a density of more than 200×106 cells/ml. Furthermore, the continuous culture of rotifer feeding on these algae was maintained. On a dry matter basis, the cultured Nannochloropsis contained 7% of EPA, and the rotifers contained 3% of EPA and 1% of docosapentaenoic acid (DPA). These results indicate that replacement of 50% of the Nannochloropsis culture water with the treated wastewater from the rotifer culture is practically feasible, and this technique can also be applied to the continuous rotifer culture.

Journal

  • Aquaculture Science

    Aquaculture Science 57 (1), 117-126, 2009

    Japanese Society for Aquaculture Science

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