Preparation of Abalone Enzymes Capable of Cell Wall Degradation of a Marine Red Alga Porphyra yezoensis (Bangiales, Rhodophyta)
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- OOTSUKA Shuuji
- Graduate School of Fisheries Sciences, Hokkaido University
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- FUKUDA Satoru
- Graduate School of Fisheries Sciences, Hokkaido University Algatech Kyowa, Kyowa Concrete Industry Co., Ltd.
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- SHIBATA Daisuke
- Graduate School of Fisheries Sciences, Hokkaido University
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- SAGA Naotsune
- Graduate School of Fisheries Sciences, Hokkaido University
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- OJIMA Takao
- Graduate School of Fisheries Sciences, Hokkaido University
Bibliographic Information
- Other Title
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- アワビ消化管由来の海産紅藻スサビノリ細胞壁分解酵素の調製
- アワビ ショウカカン ユライ ノ カイサン コウソウ スサビノリ サイボウヘキ ブンカイ コウソ ノ チョウセイ
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Abstract
We extracted a xylanase and a mannanase from Ezo abalone Haliotis discus hannai, and examined the enzyme activity and the ability for cell wall degradation of Porphyra yezoensis. Abalone xylanase was partially purified from the crude enzyme prepared from the digestive fluid by column chromatography on a TOYOPEARL CM-650M. Abalone mannanase was successfully purified from the crude enzyme by column chromatographies on TOYOPEARL CM-650M, hydroxyapatite, and TOYOPEARL HW-50E The gametophytic thalli of P.yezoensis could not be degraded by the xylanase alone. The purified mannanase could degrade the thalli into cell masses consisting of 10-20 cells; however, it produced practically no protoplasts from the thalli. When the thalli were incubated both with the xylanase and the purified mannanase, some protoplasts could be produced from the thalli. The two enzymes may be useful for the studies on cell engineering and asexual seedling production of this alga.
Journal
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- Aquaculture Science
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Aquaculture Science 54 (1), 83-88, 2006
Japanese Society for Aquaculture Science
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Details 詳細情報について
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- CRID
- 1390282679692824448
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- NII Article ID
- 10017970434
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- NII Book ID
- AN00124667
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- ISSN
- 21850194
- 03714217
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- NDL BIB ID
- 7889929
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed