マガキの正常卵および雌性発生卵における核の挙動の細胞学的観察

  • 李 〓
    Education and Research Center of Marine Bio-resources, Faculty of Agriculture, Tohoku University
  • 尾定 誠
    Education and Research Center of Marine Bio-resources, Faculty of Agriculture, Tohoku University
  • 柏原 勝
    Biomate Co., Ltd.
  • 広橋 憲
    Biomate Co., Ltd.
  • 木島 明博
    Education and Research Center of Marine Bio-resources, Faculty of Agriculture, Tohoku University

書誌事項

タイトル別名
  • Cyiological Observations on Nuclear Behavior in Normal and Gynogenetic Eggs of the Pacific Oyster, Crassostrea gigas.
  • Cytological observations on nuclear behavior in normal and gynogenetic eggs of the pacific oyster, Crassostrea gigas

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抄録

Nuclear changes in normal and gynogenetic eggs of the Pacific oyster were examined under a fluorescence microscope during meiosis and fertilization. Haploid gynogenesis was induced by sperm which were ultraviolet (UV)-irradiated for 60 s at an intensity of 72 erg/mm2/s. Although UV irradiation did not affect either meiotic maturation or the formation of the male and female pronuclei, their developmental progress was delayed. At mitotic prophase, the male pronucleus in eggs inseminated with the UV irraidated sperm formed no chromosome, unlike the female pronucleus, but became a dense chromatin body (DCB) which did not participate in the karyokinesis. At completion of cytokinesis of the first cleavage, the DCB was seen either in the cytoplasm of one of the two blastomeres or on the equatorial plate as two partitioned parts. Cytological evidence of the induction of gynogenesis in the oyster was demonstrated.

収録刊行物

  • 水産増殖

    水産増殖 48 (2), 193-198, 2000

    日本水産増殖学会

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