Effect of Brassica oxyrrhina Cytoplasm on Raphanus sativus.

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  • ダイコン(Raphanus sativus L.)におけるBrassica oxyrrhina Coss.細胞質の効果
  • ダイコン Raphanus sativus L.ニ オケル Brassica

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Brassica oxyrrhina Coss. (OxOx genone, 2n=18), a wild Brassica species was repeatedly backcrossed with radish (Raphanus sativus. L., cv. Miyashige, RR genome, 2n= 18) (Fig. 1, Table 1, 2, 3 and 4) . In the B2 generations, plants with 2n=18 chromosomes were obtained (Fig. 2). In the process of cytoplasm substitution, restoration of pollen fertility was not observed (Fig. 3). Thus, it was assumes that the depression of pollen fertility was due to the effect of B. oxyrrhina cytoplasm (Figs. 3, 5 and 6). Though the seed fertility of plants increased when their chromosome numbers were close to 2n=18, fertility was not restored up to the normal level (Fig. 4). No seeds were obtained from the plants in which pollen fertility was below 50 % and It was considered that cytoplasmic male sterility had been induced. Cytoplasm-substituted radish plants were backcrossed two times with nine commercial cultivars. Thereafter, nine plants with B. oxyrrhina cytoplasm were reciprocally crossed with each cultivar (Table 5). Two kinds of F1 plants (B. oxyrrhina-cytoplasmic F1 and R. sativus.-cytoplasmic F1) were used as materials for the comparative studies. In the Fl plants with B. oxyrrhina cytoplasm, the chlorophyll content was never reduced (Table 6). The values of root length, root diameter, root weight and leaf weight in the F1 plants of‘Akamaru hatsuka', ‘Shogoin', ‘Shijunichi' and ‘Miyashige' with B. oxyrrhina cytoplasm were lower than those of the F1 plants with R. sativus. cytoplasm. In case of F1 plants of ‘Raddike ostergruss' and ‘Nerima', there were no differences between B. oxyrrhina and R. sativus. cytoplasms (Table 6, Fig. 7). It is considered that these plants harboured a gene (or genes) compatible with the B. oxyrrhina cytoplasm. Therefore, it is suffered that the inhibition caused by the interaction between nucleus and cytoplasm may be released by hybridization.

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