Identification of Parental Chromosomes in the Interspecific Hybrids of Nicotiana rustica L.*N. tabacum L. and N. gossei Domin*N. tabacum L., Using Genomic in situ Hybridization.

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  • GISH法によるタバコ種間雑種(Nicotiana gossei Domin×N. tabacum L., N. rustica L., ×N. tabacum L.)での親由来染色体の識別
  • Identification of parental chromosomes in the interspecific hybrids of Nicotiana rustica L.×N. tabacum L.and N.gossei Domin×N.tabacum L.,using genomic in situ hybridization
  • Identification of parental chromosomes

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Abstract

Genomic in situ hybridization was carried out for mitotic cells of the interspecific hybrids of Nicotiana rustica L. (2n = 48)× N. tabacum L. (2n = 48) and N. gossei Domin (2n = 36)× N. tabacum L. Without blocking DNA of N. tabacum, clear hybridization signals from the biotinylated total genomic DNA of N. rustica or N. gossei, were obtained for the set of chromosomes, indicating that each parent could be discriminated. In the hybrid of N. rustica × N. tabacum, twenty-four chromosomes among forty-eight chromosomes were confirmed to be derived from N. rustica. Furthermore, two chromosomes exhibited regions with weak hybridization signals, suggesting that interspecific translocations might have occurred between N. rustica and N. tabacum chromosomes. On the other hand, in the hybrid of N. gossei× N. tabacum, eighteen chromosomes showing a hybridization signal over the entire length, were discriminated from the forty-two hybrid chromosomes. No region with weak signal was detected in any of them.

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