Identification and evaluation of the pathogenicity of a scuticociliate isolated from diseased barfin flounder <I>Verasper moseri</I> and sensitivity of a pathogen isolate to vinegar and tea extract solutions in sea water

Ito Shingo, Kasai Hisae

doi:10.11233/aquaculturesci.63.255

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マツカワ <I>Verasper moseri</I> から分離したスクーチカ繊毛虫の食酢・茶抽出物に対する感受性
マツカワ Verasper moseri から分離したスクーチカ繊毛虫の食酢・茶抽出物に対する感受性
マツカワ Verasper moseri カラブンリシタスクーチカセンモウチュウノショクズ・チャチュウシュツブツニタイスルカンジュセイ

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We observed mortality due to scuticociliate infection in barfin flounder and subsequently identified the causative pathogen and examined its pathogenicity. In addition, we examined the sensitivity of a pathogen isolate and the tolerance of the barfin flounder to vinegar solutions and tea extract solutions to identify scuticociliate infection control measures in this fish species. Species identification of the causative agent was performed using polymerase chain reaction (PCR); the causative agent was identified as Miamiensis avidus. M. avidus was administered to barfin flounder via immersion or intraperitoneal injection. In the intraperitoneal injection experiments, the cumulative mortality rate within one week was 100% at a dose of 10⁵ ciliates/fish. In contrast, immersion challenge experiments were unsuccessful. M. avidus survival fell below the detection limit after exposure to a 0.7% vinegar solution or a 0.01% tea extract solution for 30 min. Exposure to a 0.03% tea extract solution for 1h was toxic to the barfin flounder, whereas exposure to a 0.7% vinegar solution for 1h exhibited low toxicity. In conclusion, we suggest that exposure to a 0.7% vinegar solution for 30 min is an effective M. avidus infection treatment and control method in vitro.

Journal

Aquaculture Science

Aquaculture Science 63 (3), 255-259, 2015

Japanese Society for Aquaculture Science

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