Correlation between the Temperature Tolerance of Live Fish and Caudal Fin Cells in Black Scraper <I>Thamnaconus modestus</I>

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  • Sakamoto Kenji
    Research Institute of Marine Bioresources, Fukuyama University
  • Igarashi Mayu
    Research Institute of Marine Bioresources, Fukuyama University

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Other Title
  • ウマヅラハギの個体と尾鰭細胞における温度耐性の関連性
  • ウマズラハギ ノ コタイ ト オヒレ サイボウ ニ オケル オンド タイセイ ノ カンレンセイ

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Abstract

We evaluated the correlation between the temperature tolerance of live fish in vivo and caudal fin cells in vitro in black scraper Thamnaconus modestus. Fish were reared at 17°C then exposed to high temperature (30°C) and the time to death was measured. In addition, we incubated black scraper caudal fin cells at 43°C for 2 h and measured cell viability using the trypan blue assay. There was a significant correlation between the in vivo and the in vitro tolerance to high temperature in this species (P<0.05). Our results suggest that the caudal fin cells from live fish may be used to evaluate high temperature tolerance traits in selective fish breeding programs.

Journal

  • Aquaculture Science

    Aquaculture Science 62 (1), 31-35, 2014

    Japanese Society for Aquaculture Science

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