マイクロプレートを用いたアコヤガイの人工授精方法
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- Ohta Hiromi
- Department of Fisheries, Faculty of Agriculture, Kinki University
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- Arita Kahori
- Department of Fisheries, Faculty of Agriculture, Kinki University
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- Isowa Kiyoshi
- Mie Prefectural Fish Farming Center
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- Ishikawa Takashi
- Faculty of Bioresources, Mie University
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- Aoki Hideo
- Mie Prefecture Fisheries Research Institute
書誌事項
- タイトル別名
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- Development of an Artificial Fertilization Method for the Japanese Pearl Oyster <I>Pinctada fucata martensii</I> by Using a Microplate
- Development of an artificial fertilization method for the Japanese pearl oyster Pinctada fucata martensii by using a microplate
この論文をさがす
抄録
Japanese pearl oysters are widely cultivated by artificial fertilization using gametes obtained from the ovary and testis. Using the gametes from the Japanese pearl oyster, we developed a method for conducting small-scale artificial fertilization tests in a 24-well microplate. Following induction of germinal vesicle breakdown (GVBD), we inseminated 20,000 eggs in 2.0 ml seawater in a single well of a microplate. The highest fertilization rates were obtained using a testicular semen volume of 1.25μl when the concentration of NH3 in seawater was 0.75 mM during insemination. Changes in contact time (the time from commencement of insemination to rinsing the eggs) from 3 to 60 min did not affect the fertilization rate. However, a contact time of >12 min was necessary to ensure consistency in contact time among the 24 wells.
収録刊行物
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- 水産増殖
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水産増殖 58 (4), 517-523, 2010
日本水産増殖学会
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詳細情報 詳細情報について
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- CRID
- 1390282679697139456
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- NII論文ID
- 10029847027
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- NII書誌ID
- AN00124667
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- ISSN
- 21850194
- 03714217
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- NDL書誌ID
- 10954557
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可