Molecular cloning of cDNAs encoding multiple vitellogenins in Amur sturgeon <I>Acipenser schrenckii</I>

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  • アムールチョウザメ <I>Acipenser schrenckii</I> の多型ビテロジェニン遺伝子のcDNAクローニング
  • アムールチョウザメAcipenser schrenckiiの多型ビテロジェニン遺伝子のcDNAクローニング
  • アムールチョウザメ Acipenser schrenckii ノ タケイ ビテロジェニン イデンシ ノ cDNA クローニング

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Abstract

Molecular cloning of cDNAs encoding an egg yolk precursor, vitellogenin (Vtg), was performed using the liver of estrogen-treated Amur sturgeon. Three kinds of vtg cDNA were cloned and temporarily named as vtg1, vtg2 and vtg3. These vtg cDNAs were obtained as contiguous sequences; each of vtg1, vtg2 and vtg3 sequences contained a complete open reading frame (5,307, 5,247 and 5,319 bp, respectively), each encoding 1,769, 1,749 and 1,773 aa residues, respectively. Similarity among amino acid sequences deduced from the three vtg cDNAs were relatively low, ranging from 64.3% to 47.9%. Three Vtgs appeared to be a complete-type, consisting of all representative yolk protein domains. Phylogenetic analysis revealed that Amur sturgeon Vtg1 formed a clade together with a published white sturgeon Vtg, while Amur sturgeon Vtg2 and Vtg3 formed another distinct clade. The results of phylogenetic analysis confirmed all three Vtgs belong to VtgAB type; vtg1/Vtg1, vtg2/Vtg2 and vtg3/Vtg3 were hereby designated as vtgAB1/VtgAB1, vtgAB2a/VtgAB2a and vtgAB2b/VtgAB2b, respectively. This study provided a basis to understand multiplicity in sturgeon vtg/Vtg and set a stage for their future application as reproductive biomarkers in sturgeon aquaculture.

Journal

  • Aquaculture Science

    Aquaculture Science 64 (1), 63-76, 2016

    Japanese Society for Aquaculture Science

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