アグロバクテリウムを用いたトレニア形質転換体の作出

間 竜太郎, 柴田 道夫

doi:10.1270/jsbbs1951.45.71

アグロバクテリウムを用いたトレニア形質転換体の作出

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書誌事項

タイトル別名

Agrobacterium-mediated Transformation of Torenia (Torenia fournieri).
アグロバクテリウムを用いたトレニア形質転換体の作出〔英文〕
アグロバクテリウムオモチイタトレニアケイシツテンカンタイノサクシ

公開日: 1995

DOI

10.1270/jsbbs1951.45.71

公開者: 日本育種学会

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説明

A method for Agrobacterium -mediated transformation of torenia (Scrophulariaceae) is reported. Leaf segments of torenia plants grown in vitro were infected with the Agrobacterium strain LBA4404 harboring a binary vector containing the neomycin phosphotransferase IIgene, P -glucuronidase (GUS) gene lvith an intron and hygromycin phosphotransferase gene. The infected explants were cultured on Murashige and Skoog medium supplemented with 1 mg/l benzyladenine, 100 mgfl carbenicillin and 300 mg/l kanamycin (selection medium) for regeneration. Twelve shoots out of 67 shoots which regenerated from green compact calli on the selection medium were GLTS-positive. Polymerase chain reaction analysis confirmed the presence of the GUS gene in the GUS-positive plants. Mendelian inheritance of the GUS activity in the progenies of the transformed plants was demonstrated.