Development of plants resistant to Papaya leaf distortion mosaic virus by intergeneric hybridization between Carica papaya and Vasconcellea cundinamarcensis
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- Tarora Kazuhiko
- Okinawa Prefectural Agricultural Research Center Department of Bioscience and Textile Technology, Shinshu University
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- Shudo Ayano
- Okinawa Prefectural Agricultural Research Center
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- Kawano Shinji
- Department of Agriculture, Forestry and Fisheries, Yaeyama Agriculture, Forestry and Fisheries Promotion Center
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- Yasuda Keiji
- Okinawa Prefectural Forest Resources Research Center
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- Ueno Hiroki
- Department of Bioscience and Textile Technology, Shinshu University Institute of Vegetable and Floriculture Science, NARO
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- Matsumura Hideo
- Gene Research Center, Shinshu University
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- Urasaki Naoya
- Okinawa Prefectural Agricultural Research Center
書誌事項
- タイトル別名
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- Development of plants resistant to <i>Papaya leaf distortion mosaic virus</i> by intergeneric hybridization between <i>Carica papaya</i> and <i>Vasconcellea cundinamarcensis</i>
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<p>In this study, we confirmed that Vasconcellea cundinamarcensis resists Papaya leaf distortion mosaic virus (PLDMV), and used it to produce intergeneric hybrids with Carica papaya. From the cross between C. papaya and V. cundinamarcensis, we obtained 147 seeds with embryos. Though C. papaya is a monoembryonic plant, multiple embryos were observed in all 147 seeds. We produced 218 plants from 28 seeds by means of embryo-rescue culture. All plants had pubescence on their petioles and stems characteristic of V. cundinamarcensis. Flow cytometry and PCR of 28 plants confirmed they were intergeneric hybrids. To evaluate virus resistance, mechanical inoculation of PLDMV was carried out. The test showed that 41 of 134 intergeneric hybrid plants showed no symptoms and were resistant. The remaining 93 hybrids showed necrotic lesions on the younger leaves than the inoculated leaves. In most of the 93 hybrids, the necrotic lesions enclosed the virus and prevented further spread. These results suggest that the intergeneric hybrids will be valuable material for PLDMV-resistant papaya breeding.</p>
収録刊行物
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- Ｂｒｅｅｄｉｎｇ Ｓｃｉｅｎｃｅ
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Ｂｒｅｅｄｉｎｇ Ｓｃｉｅｎｃｅ 66 (5), 734-741, 2016
日本育種学会
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詳細情報 詳細情報について
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- CRID
- 1390282679698431488
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- NII論文ID
- 130005290474
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- NII書誌ID
- AA11317194
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- ISSN
- 13473735
- 13447610
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- NDL書誌ID
- 027816589
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- 本文言語コード
- en
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- 資料種別
- journal article
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