Homology-dependent Suppression of Stigma Phenotype by an Antisense S-locus Glycoprotein (SLG) Gene in Brassica rapa L.
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- Takasaki Takeshi
- Research Institute of Seed Production Co., Ltd. Faculty of Agriculture, Kobe University
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- Hatakeyama Katsunori
- Research Institute of Seed Production Co., Ltd.
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- Watanabe Masao
- Faculty of Agriculture, Iwate University
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- Toriyama Kinya
- Graduate School of Agricultural Sciences, Tohoku University
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- Hinata Kokichi
- Research Institute of Seed Production Co., Ltd.
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Self-incompatibility (SI) in Brassicaceae is sporophytically controlled by haplotypes of the polymorphic S locus complex. Two tightly linked polymorphic genes at the S locus, S-locus glycoprotein (SLG) and S-receptor kinase (SRK) genes, are specifically expressed in the stigma. S-haplotypes have been classified into class I and class II types based on the sequence similarity of their SLGs, and their SRKs. To investigate the effect of an antisense SLG gene on the class divergency of the endogenous SLG and SRK genes, we introduced an antisense class I SLG43 cDNA into a cultivar Osome in Brassica rapa which was heterozygous for class I S52 haplotype and class II S60. SLG43 is more similar to the endogenous SLG52 (87.8% identity) than to SLG60 (74.8% identity), Out of ten primary transformants analyzed, two were completely self-compatible; the SI phenotype of stigma was altered from S52S60 to S60, but that of pollen was not. In these two plants, the expression levels of mRNA and protein of SLG52 were reduced, whereas those of SLG60 were not. We suggest that an antisense class I SLG-transgene causes homology-dependent suppression, which leads to breakdown of the class I S-haplotype specificity in stigma but not the class II S-haplotype.
収録刊行物
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- Breeding Science
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Breeding Science 51 (2), 89-94, 2001
日本育種学会
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詳細情報 詳細情報について
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- CRID
- 1390282679698433152
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- NII論文ID
- 130004145842
- 110001808085
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- NII書誌ID
- AA11317194
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- ISSN
- 13473735
- 13447610
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- HANDLE
- 10097/46805
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- NDL書誌ID
- 5791639
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 使用不可