Study on Potentialization of FOM and cephems(SBT/CPZ and CAZ) on P. aeruginosa.

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  • 緑膿菌に対するホスホマイシンとセフェム剤 (スルバクタムセフォペラゾンとセフタジジム) の併用効果の検討

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The purpose of this study was to investigate of sulbactam/cefoperazon (SBT/ CPZ) and ceftazidim (CAZ) on 4 strains of P. aeruginosa when they are used with fosfomycin (FOM) both in vivo and in vitro.<BR>MICs of the 3 agents against 4 strains were measured. The producibility of β-lactamase against 4 strains was checked. To investigate the combined use of FOM with the Cephems, FTC (Fractional Inhibitory Concentration) indexes were calculated by means of the checker board method.<BR>After MLD (Minimal Lethal Dose) of each strain for a mouse was calculated, twice the amount of MLD was given to each of the 6 mice by intraperitoneal injection.<BR>Two hours later, each of the mice was administered single antimicrobial agent and ED50 was determined. The Concentration ratio of FOM to SBT/CPZ as well as FOM to CAZ was set as follows based on these ED50 : 1 : 1, 1/2: 1/2, 1/4: 1/4, 1/8: 1/8, 1/2: 1/4, 1/2: 1/8, and 1/2: 1/16.<BR>The survival of the mice was observed after 15 hours, 24 hours, 48 hours and one week.<BR>Every strain could produce β-lactamase. MICs of SBT/CPZ were 12.5 25μg/ml ; MICs of CAZ were 1.56-3.13/μg/ml ; and MICs of FOM were 50-200μg/ml. The sensitivity of CAZ was superior to that of the others. As to FTC index of SBT/CPZ + FOM, 3 strains had 0.375 and 1 strain had 0.75. As to CAZ + FOM, 1 strain had 0.375, 2 strains had 0.5, and 1 strain had 0.75, which revealed that SBT/CPZ + FOM had a greater potentialization. CAZ, when used alone, was superior in the ED50 investigation. However, when used with FOM, SBT/CPZ showed better results.

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