SUICIDE GENE THERAPY WITN AN ADENOVIRUS VECTOR CONTAINING THE HERPES SIMPLEX VIRUS THYMIDINE KINASE GENE FOR HEAD AND NECK SQUAMOUS CELL CARCINOMA IN VIVO

DOI 12 References
  • FUKUHARA Hirokazu
    Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine
  • HAYASHI Yasushi
    Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine
  • FUKUI Takafumi
    Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine
  • YAMAMOTO Noriyuki
    Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine
  • KAGAMI Hideaki
    Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine
  • TOHNAI Iwai
    Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine
  • UEDA Minoru
    Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine
  • MIZUNO Masaaki
    Department of Molecular Neurosurgery, Nagoya University Graduate School of Medicine
  • YOSHIDA Jun
    Department of Neurosurgery, Nagoya University Graduate School of Medicine

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Other Title
  • 口腔癌に対するアデノウイルスベクターを用いた自殺遺伝子治療

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Abstract

The purpose of this study was to evaluate the usefulness of suicide gene therapy with an adenovirus (Ad) vector for human head and neck squamous cell carcinoma (HNSCC) cells.<br>First, we investigated the transduction efficiencies and the rate of cancer cell death with suicide gene therapy four HNSCC cell line HSC-2, HSC-3, HSC-4, and SAS. The transduction efficiencies were about 15% at an MOI of 1, and almost 100% at an MOI of 10, in vitro with AdlacZ. The gene transduction with Adtk following administration of GCV induced cancer cell death. The rates of cancer cell death were about 70% at an MOI of 1, and almost 100% at an MOI of 10, in vitro.<br>Next, we observed the gene transduction into tumor that was transplanted into a nude mouse, and we then evaluated the anti-tumor effects of suicide gene therapy in vivo. There a was statistical difference in the anti-tumor effect between the treatment group and the control group. Immunohistochemical staining did not show the development of anti-tumor immunity with suicide gene therapy.<br>These results indicate that suicide gene therapy with an Ad vector is effective for HNSCC cells. Clarification of the mechanism of the bystander effect will be required to intend the reinforcement of suicide gene therapy.

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