Target cell recognition of anti-CD3 antibody and IL-2 induced LAK cells from lymphocytes on patients with oral squamous cell carcinoma.

DOI
  • MORI Yoshiyuki
    The second department of oral and maxillofacial surgery, Faculty of dentistry, Tokyo medical and dental university
  • TAKAHASHI Yuzo
    The second department of oral and maxillofacial surgery, Faculty of dentistry, Tokyo medical and dental university
  • MINATO Shuji
    The second department of oral and maxillofacial surgery, Faculty of dentistry, Tokyo medical and dental university
  • FUJIBAYASHI Takashi
    The second department of oral and maxillofacial surgery, Faculty of dentistry, Tokyo medical and dental university
  • ENOMOTO Shoji
    The second department of oral and maxillofacial surgery, Faculty of dentistry, Tokyo medical and dental university

Bibliographic Information

Other Title
  • 口腔へん平上皮癌患者リンパ球より誘導された抗ＣＤ３抗体／ＩＬ‐２活性化細胞（ＣＤ３・ＬＡＫ）の標的細胞認識の検索

Description

We investigated the characteristics and target cell recognition of CD3·LAK induced by anti-CD3 monoclonal antibody (mAb) and interleukin 2 (IL-2) from peripheral blood lymphocytes (PBL) on the patients with oral squamous cell carcinoma.<br>‹Materials and Methods› CD3·LAK were induced by 10μg/ml of anti-CD3mAb (solid phase) and 700JRU/ml of IL-2 (S6820). Surface marker of CD3·LAK were detected by flow cytometry. A 4-h51Cr release assay was used to detect cytotoxic activity. Cultured tumor cell lines, which are Ca9-22 (established from squamous cell carcinoma of the lower gingiva: case 1), NA (established from squamous cell carcinoma of the tongue) and K562, were used as target cells. The expression of HLA antigen on the surface of the cultured tumor cell lines were detected by flow cytometry prior to 4-h 51Cr releas eassay detection. In order to investigate the target cell recognition of CD3·LAK, inhibition assay with mAb were done.<br>‹Results› On day 19, CD3·LAK population mainly consisted of TCR α/β positive T cell (CD3+) and NK cell (CD3-NKH-1+), and remaining portion was TCR γ/δ positive T cell (CD3+). The CD3·LAK showed the cytotoxic activities against autologous and allogeneic cultured tumor cell lines, and these activities were inhibited by anti-CD3 monoclonal antibody. Ho wever the cytotoxic activities against K562 were not inhibited by anti-CD3 monoclonal antibody. These results indicate that the target cell recognition of CD3·LAK does not need MHC-restriction. CD3·LAK maybe concerned with two different types of target cell recognition which can either be through TCR/CD3 complex or none at all.

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Keywords

Details 詳細情報について

  • CRID
    1390282679706245632
  • NII Article ID
    130004166356
  • DOI
    10.5981/jjhnc1974.18.2_120
  • ISSN
    18839878
    09114335
  • Text Lang
    ja
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
  • Abstract License Flag
    Disallowed

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