ＧＣ‐ＭＳおよびＵＶ法を用いたヒト乳癌細胞（ＺＲ‐７５‐１）の脂質過酸化と細胞死の解析 ｇａｍｍａ‐ｌｉｎｏｌｅｎｉｃ ａｃｉｄと鉄イオンに対する反応
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- 武田 俊平
- Efamol Research Institute
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- Horrobin David
- Efamol Research Institute
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- Sim Greig
- Institute for Marine Biosciences, National Research Council Canada
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- Sanford Tim
- Efamol Research Institute
書誌事項
- タイトル別名
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- GC-MS and UV-spectrophotometric investigation of accelerated lipid peroxidation and cell destruction in cultured human breast cancer cells (ZR-75-1) produced by gammalinolenic acid and iron.
説明
Lipid peroxidation and its anticancer effect induced by gamma-linolenic acid and iron (GLA+Fe) were studied by ultraviolet-spectrophotometric (UV) and gas chromatographic-mass spectrometric (GC-MS) methods. When both were supplemented with GLA +Fe, there were far more dead cells in human breast cancer (ZR-75-1) cultures than in human normal skin fibroblasts (CCD 41-Sk) . Accelerated lipid peroxidation was confirmed by the formation of conjugated dienes (CD) in the polyunsaturated fatty acids (PUFAs) and by the formation of hydroperoxyl group (OOH) indicated by the conversion of triphenylphosphine (TPP) to its oxide (TPPO) only in ZR-75-1 cells but not in CCD 41-Sk cells treated with GLA+Fe. Vitamin E inhibited the enhancement of peroxidation and cell killing produced by Fe in ZR-75-1 cells. It was shown that an accelerated production of lipid peroxides detected as CD and OOH-TPP reaction in cancer cells (ZR-75-1) was closely associated with the cancer cell killing produced by GLA+Fe.<BR>These findings suggest a lipid peroxidation mechanism specific to malignant cells to be investigated further, which might open the possibility of applying PUFA such as GLA as a highly selective antineoplastic agent and a sensitive reagent to detect malignancy.
収録刊行物
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- 炎症
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炎症 13 (1), 27-34, 1993
日本炎症・再生医学会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390282679761488000
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- NII論文ID
- 130003856296
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- ISSN
- 18844006
- 03894290
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可