Analysis of aflatoxin B<SUB>1</SUB>-human serum albumin adducts by a sandwich enzyme-linked immunosorbent assay

DOI Open Access
  • KAWAMURA Osamu
    Faculty of Pharmaceutical Sciences, Science University of Tokyo
  • LIM J.-M.
    Faculty of Pharmaceutical Sciences, Science University of Tokyo
  • OKUMURA Hiroki
    Faculty of Pharmaceutical Sciences, Science University of Tokyo
  • KISHIMOTO Sigefumi
    Faculty of Pharmaceutical Sciences, Science University of Tokyo
  • CHEN G.
    Institute of Public Health, Shanghai Medical University
  • YU S.-Z.
    Institute of Public Health, Shanghai Medical University
  • UENO Yoshio
    Faculty of Pharmaceutical Sciences, Science University of Tokyo

Bibliographic Information

Other Title
  • サンドイッチELISAによるアフラトキシンB<SUB>l</SUB>-ヒト血清アルブミン付加体測定
  • Analysis of aflatoxin B1-human serum albumin adducts by a sandwich enzyme-linked immunosorbent assay

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Description

Employing monoclonal antibody (mAb) AL. 2 reactive for aflatoxin B1-lysin adducts (AFB1-Lys), biotin-conjugated affinity purified anti-human serum albumin (HSA) antibody, and streptoavidin-conjugated horseradish peroxidase (HRP), the sandwich ELISA (sELISA) AFB1-HSA adducts was constructed. In this sELISA, AFB1-HSA adducts in crude albumin fraction were detected as low as 5pg AFB1-Lys equivalent/mg HSA without pronase digestion. Since the present sELISA is not requested for an enzymatic hydrolysis of HSA, more than 150 serum samples could be analyzed in one week. AFB1-HSA analysis of 70 human sera sampled during 1986-1994 in Haimen (China), a high endemic area of primary liver cancer (PLC), revealed that 20-70% of the sera were positive for AFB1-HSA adducts. The case-control study on 137 sera (69 controls and 68 cases of PLC) collected in 1994 revealed no significant difference both in the positivity and the average level. As for 100 sera (44 controls and 56 cases) sampled in 1995, the postivity for AFB1-HSA in the cases was significantly higher than that in the controls, although no difference in their contents was observed.

Journal

  • JSM Mycotoxins

    JSM Mycotoxins 1996 (43), 43-46, 1996

    Japanese Society of Mycotoxicology

Keywords

Details 詳細情報について

  • CRID
    1390282679762004480
  • NII Article ID
    130003446764
  • DOI
    10.2520/myco1975.1996.43_43
  • ISSN
    18810128
    02851466
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • Abstract License Flag
    Disallowed

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