Studies on mitogen-induced proliferative response in colorimetric MTT assay.

DOI 1 Citations
  • Kobayashi Toyokazu
    Division of Rheumatology, Department of Medicine, Juntendo University School of Medicine
  • Harada Hitomi
    Laboratory for Pharmacological Research, Institute For Life Science Research, Asahi Chemical Industry Co., LTD.
  • Shibata Kensuke
    Laboratory for Pharmacological Research, Institute For Life Science Research, Asahi Chemical Industry Co., LTD.
  • Hirose Shun-ichi
    Division of Rheumatology, Department of Medicine, Juntendo University School of Medicine

Bibliographic Information

Other Title
  • ＭＴＴ法によるリンパ球幼若化反応の検討

Search this article

Description

The effect of several agents on mitogen-induced proliferative responsein vitrowas studied by using colorimetric MTT [3- (4, 5-dimenthyl-2-thiazolyl) -2, 5-diphenyl-2H tetrazolium bromide] assay. Immuno-modulator levamisole and TOK-8801 enhanced concanavalin A and lipopolysaccharide response in murine splenocytes at concentrations of 10-6-10-5M and 10-4M, respectively. Immunosuppressant mizoribine decreased dose dependently this response.<BR>These results show that MTT assay may be a useful method for the evaluation of immunological affecting agents on cell proliferative response.

Journal

  • Ensho

    Ensho 13 (4), 383-386, 1993

    The Japanese Society of Inflammation and Regeneration

Citations (1)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top