Application of silica extracted from rice husk ash for the encapsulation of AFB<sub>1</sub> antibody as a matrix in immunoaffinity columns

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  • Pranowo Deni
    Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada
  • Nuryono
    Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada
  • Agus Ali
    Department of Animal Science and Husbandry, Faculty of Animal Husbandry, Universitas Gadjah Mada
  • Jumina
    Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada
  • Maryam Romsyah
    Toxicology and Mycology Research Group, Indonesian Research Centre for Veterinary Science
  • Setyabudi FMCS
    Department of Food and Agricultural Product Technology, Faculty of Agriculture Technology, Universitas Gadjah Mada

Bibliographic Information

Other Title
  • コメ籾殻灰由来シリカのイムノアフィニティカラムにおけるAFB<sub>1</sub>抗体封入剤としての活用
  • Application of silica extracted from rice husk ash for the encapsulation of AFB₁ antibody as a matrix in immunoaffinity columns

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Abstract

<p>  The extraction of silica from rice husk ash (RHA) for the encapsulation of aflatoxin B1 antibody (Ab-AFB1) and its application as a matrix in immunoaffinity columns (IACs) were achieved. The RHA extraction was performed using 4 M NaOH, which yielded sodium silicate (Na2SiO3) for the synthesis of silica gel. The obtained silica was used for encapsulating Ab-AFB1 using the sol-gel technique. One milliliter of 1 M Na2SiO3:H2O:H3PO4 (0.43:0.11:0.46) could generate silica gel that was suitable for encapsulating 1.36 mg of Ab-AFB1 at pH 7. After 48 hours of aging, the silica gel modified with Ab-AFB1 (SG-Ab-AFB1) was ground, and packed as the matrix in the IAC for aflatoxins purification. The modified silica gel was characterized using FTIR and SEM. The properties of IAC with SG-Ab-AFB1 were investigated by evaluating AF recovery, binding capacity, and reusability. The recovery of AFB1 was 94.11 ± 4.62%. In addition to AFB1 recovery, the column also retained AFB2, AFG1, and AFG2 with recovery values of 98.22 ± 3.74%, 92.22 ± 7.62%, and 83.00 ± 6.31%, respectively. This column, which contained 0.5 g of SG-Ab-AFB1 had a binding capacity of approximately 50 ng of AFs per column, and could be reused at least 5 times with a recovery of more than 80%.</p>

Journal

  • JSM Mycotoxins

    JSM Mycotoxins 67 (2), 77-83, 2017

    Japanese Society of Mycotoxicology

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