Functional screening for resistance genes against trichothecenes in the library of <i>Saccharomyces cerevisiae</i> deletion mutants

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  • TAKAHASHI-ANDO Naoko
    Graduate School of Engineering, Toyo University Bio-Nano Electronics Research Centre, Toyo University
  • TANAKA Akira
    Graduate School of Engineering, Toyo University
  • SEKIMOTO Yohsuke
    Graduate School of Engineering, Toyo University
  • YAMAUCHI Kohta
    Graduate School of Engineering, Toyo University
  • ECHIGO Akinobu
    Bio-Nano Electronics Research Centre, Toyo University
  • USAMI Ron
    Graduate School of Engineering, Toyo University Bio-Nano Electronics Research Centre, Toyo University
  • ABE Fumiyoshi
    Department of Chemistry and Biological Science, College of Science and Engineering, Aoyama Gakuin University
  • MINEGISHI Hiroaki
    Bio-Nano Electronics Research Centre, Toyo University

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Other Title
  • <i>Saccharomyces cerevisiae</i> の遺伝子破壊ライブラリーを利用したトリコテセン耐性遺伝子の探索
  • Functional screening for resistance genes against trichothecenes in the library of Saccharomyces cerevisiae deletion mutants

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Abstract

  Trichothecenes are mycotoxins produced by various species of fungi, including Fusarium species. They contaminate important crops such as wheat and corn, and cause toxicity in animals and humans mainly by inhibition of protein synthesis. In this study, we performed genome-wide screening of genes that play roles in protection of Saccharomyces cerevisiae against A-type trichothecene, T-2 toxin, and D-type trichothecene, verrucarin A. Although a distinct difference exists in the structures of these two trichothecenes, these toxins inhibited the growth of the mutant strains of S. cerevisiae with common disrupted genes. The genes whose deletion conferred high sensitivity to these trichothecenes were considered as trichothecene resistance genes, and they included the ones encoding ABC transporter protein, enzymes for ergosterol biosynthesis, vacuolar H+-ATPase, and others. These results possibly lead to the construction of a yeast detection system with improved sensitivity to trichothecenes.

Journal

  • JSM Mycotoxins

    JSM Mycotoxins 63 (1), 9-15, 2013

    Japanese Society of Mycotoxicology

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