A promoter of <i>Fusarium graminearum</i> <i>Tri4</i> does not function when placed at the end of the trichothecene gene cluster
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- TOKAI Takeshi
- Plant & Microbial Metabolic Engineering Research Unit, DRI, RIKEN
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- NAKAJIMA Yuichi
- Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
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- ICHIKAWA Hinayo
- Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
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- MAEDA Kazuyuki
- Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
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- NISHIUCHI Takumi
- Division of Functional Genomics, Advanced Science Research Center, Kanazawa University
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- KOBAYASHI Tetsuo
- Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
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- KIMURA Makoto
- Plant & Microbial Metabolic Engineering Research Unit, DRI, RIKEN Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
Bibliographic Information
- Other Title
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- <i>Fusarium graminearum</i> の <i>Tri4</i> 遺伝子のプロモーターをトリコテセン生合成遺伝子クラスターの末端に配置すると機能しない
- A promoter of Fusarium graminearum Tri4 does not function when placed at the end of the trichothecene gene cluster
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Abstract
The central region of the trichothecene gene cluster contains two pathway and two regulatory genes (four Tri genes) that are necessary for forming the trichothecene skeleton. One of these genes, Tri4, encodes a multifunctional cytochrome P 450 monooxygenase, and the expression of this gene is highly induced under trichothecene-inducing conditions. Apart from this gene cluster, Tri101 occurs as a single non-cluster gene, and the product of this gene contributes to the function of self-protection in Fusarium graminearum. The promoter activity of these Tri genes was compared with that of TEF1α (a highly expressed translation elongation factor 1-alpha) gene under toxin-inducing conditions by using the β-glucuronidase (GUS) gene as a reporter. The Tri101 promoter- and TEF1α promoter-reporter fusions integrated at the end of the gene cluster showed activities that were similar to those of the original loci with respect to the timing and the level of GUS protein accumulation. However, as opposed to the normal pattern of Tri4 transcription from the original locus, no GUS activity was detected when the Tri4 promoter was placed at the end of the gene cluster. Thus, we can conclude that the position of the promoter in the gene cluster is important for native transcriptional regulation of Tri4 in trichothecene biosynthesis.
Journal
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- JSM Mycotoxins
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JSM Mycotoxins 63 (1), 17-25, 2013
Japanese Society of Mycotoxicology
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Keywords
Details 詳細情報について
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- CRID
- 1390282679765071488
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- NII Article ID
- 10031161943
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- NII Book ID
- AN00334513
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- COI
- 1:CAS:528:DC%2BC3sXhtVGit7%2FM
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- ISSN
- 18810128
- 02851466
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- NDL BIB ID
- 027191461
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed