書誌事項
- タイトル別名
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- DNA Histogram of Neuroblastoma Flow-microfluorometric Study
- シンケイガシュ ノ DNA ヒストグラム
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説明
Eight neuroblastomas and 1 ganglioneuroma of human subjects, 1 serial transplanted neuroblastoma in nude mice and C-1300 mouse neuroblastoma cell line were studied on its cell kinetics by flow-microfluorometry (FMF). The specimens resected from stage IV neuroblastoma patients and nude mouse were freezen in liquid nitrogen. The tumor of about 1 cm^3 block was minced and tumor cell suspensions were prepared by treatment with 0.25% Trypsin solution. Following fixation by 50% ethanol, Propidium Iodide staining was carried and DNA histogram was obtained by FMF (4800A biophisics U.S.A.). DNA histograms were divided into three different types ; unimodal, bimodal and intermediate types. Furthermore, cell population ratio of near 2C compartment were calculated. About 4×10^3 to 3×10^4 of cells were measured. Control cells were chicken red blood cells and human lymphocytes which showed 2C unimodal pattern. Five of 8 cases showed near 2C unimodal pattern. The other neuroblastomas and a ganglioneuroma were with intermediate pattern. Serial transplanted tumor in nude mice was with bimodal pattern. C-1300 mouse neuroblastoma cell line was with multimodal pattern. The specimens excised after high dose cyclophosphamide therapy showed relatively low population of 2C compartment cells compared with non-treated cases. It is still not clear how cell cycle of neuroblastoma changes by various therapeutic methods. This cytofluorometric analysis is expected to make therapeutic schedules more reasonable and effective.
収録刊行物
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- Journal of the Japanese Society of Pediatric Surgeons
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Journal of the Japanese Society of Pediatric Surgeons 17 (2), 279-282, 1981
特定非営利活動法人 日本小児外科学会
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詳細情報 詳細情報について
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- CRID
- 1390282679780145536
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- NII論文ID
- 110002136777
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- NII書誌ID
- AN00192281
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- ISSN
- 21874247
- 0288609X
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- NDL書誌ID
- 2349916
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可