Determination of Plasma Total Renin Concentration as a Marker for Wilms' Tumor : Value of RIA With a Monoclonal Antibody

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  • ウイルムス腫瘍の腫瘍マーカーとしての血漿総レニン濃度の測定 : モノクローナル抗体による RIA 法での検討

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Abstract

No tumor marker specific for Wilms' tumor has been known until recently. But according to recent studies, plasma total renin can be a new, useful marker for Wilms' tumor. Total renin consists of inactive renin and active renin, and it is known that inactive renin accounts for 70 to 90% of total renin in plasma. The conventional method for determining the level of total renin is complicated. In order to develop a simpler technique, a monoclonal antibody specific for both active renin and inactive renin was made, and a radioimmunoassay system was established. Our RIA system has been applied to 133 samples from 89 patients, including 22 Wilms' tumor, 10 neuroblastoma, 7 other tumors, and 50 controls. Plasma total renin levels were also determined with the conventional method. The coefficient of the data obtained by these two different techniques was 0.921. Plasma total renin levels in patients with Wilms' tumor were significantly increased (391.8±287.0pg/ml), as compared with those in controls (165.8±67.5pg/ml, p<0.01). After removal of Wilms' tumors, they all decreased to the normal range. It is therefore concluded that plasma total renin level determied with a monoclonal antibody is useful marker in Wilms' tumor.

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