HISTOLOGICAL ANALYSIS OF THE IMMUNOLOGICAL ASPECT IN BLADDER CARCINOMA TISSUES

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  • ISHIKAWA Kimiyasu
    Department of Urology, Showa University, School of Medicine
  • OGAWA Yoshio
    Department of Urology, Showa University, School of Medicine
  • MORITA Jun
    Department of Urology, Showa University, School of Medicine
  • ASO Takayuki
    Department of Urology, Showa University, School of Medicine
  • NAOE Michio
    Department of Urology, Showa University, School of Medicine
  • FUJI Kohzo
    Department of Urology, Showa University, School of Medicine
  • YOSHIDA Hideki
    Department of Urology, Showa University, School of Medicine
  • KUSHIMA Miki
    Department of Pathology, Showa University Hospital

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Other Title
  • 担癌膀胱における局所免疫環境の組織学的検討
  • タンガン ボウコウ ニ オケル キョクショ メンエキ カンキョウ ノ ソシキガクテキ ケントウ

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Abstract

(Objectives) To evaluate the immunological aspect of bladder cancer, we assessed infiltrating cytotoxic-T cells (CD8 positive cells), mature dendritic cells (CD83 positive cells), helper-T cells (CD4 positive cells) and the expression level of transforming growth factor-beta (TGF-beta) in bladder cancer tissues, as well as in the surrounding normal counterpart. (Materials and methods) 19 FFPE (formalin fixed paraffin embedded) bladder cancer specimens derived from patients who had undergone total cystectomy were used for this study. All 19 specimens contained bladder cancer and a normal counterpart. Immunohistochemical analyses for CD4, CD8, CD83 and TGF-beta were performed with the conventional ABC method. Cells positive for CD4, CD8 and CD83 were counted using an ocular grid. In addition, the expression level of TGF-beta was measured with an image analyzer. (Result) Cells positive for CD8 were observed more predominantly in the normal counterpart than in cancer tissues. The staining intensity of TGF-beta within the cancer area was significantly higher than that of the normal counterpart. The number of cells positive for CD8 in the normal counterpart was significantly higher than that of the cancer area. In this study, it was hard to estimate cells positive for CD4 and CD83 because the number of these types of cells in the bladder tissues was very low. The lower number of cells positive for CD8 and up-regulated TGF-beta in cancer tissues may indicate that bladder cancer evades immune surveillance. (Conclusions) A lower number of CD8 positive cells and up-regulated TGF-beta in a cancer area may indicate that an immunological evasion related to bladder cancer.

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