SEMIQUANTITATIVE ANALYSIS OF THE ABSORPTION-ELUTION TEST FOR ABO BLOOD GROUPING OF BLOODSTAINS
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- IZAWA Hikaru
- Department of Legal Medicine, Showa University School of Medicine
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- UMEZAWA Hironobu
- Department of Legal Medicine, Showa University School of Medicine
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- FUJISHIRO Masaya
- Department of Legal Medicine, Showa University School of Medicine
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- ISHIWATA Yasuhiro
- Department of Legal Medicine, Showa University School of Medicine
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- OHTAWA Takeyuki
- Department of Legal Medicine, Showa University School of Medicine
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- ARIMA Yoshiko
- Department of Legal Medicine, Showa University School of Medicine
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- TAKAHASHI Yoshiharu
- Department of Legal Medicine, Showa University School of Medicine
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- LEE Xiao-Pen
- Department of Legal Medicine, Showa University School of Medicine
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- TSUTSUMI Hajime
- Department of Legal Medicine, Showa University School of Medicine
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- SATO Keizo
- Department of Legal Medicine, Showa University School of Medicine
Bibliographic Information
- Other Title
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- 解離試験による血痕のABO式血液型判定法の半定量的解析
- カイリ シケン ニ ヨル ケッコン ノ ABOシキ ケツエキガタ ハンテイホウ ノ ハンテイリョウテキ カイセキ
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Abstract
To date, the absorption-elution test has been widely used for ABO blood grouping of bloodstains, hairs, nails, and teeth. Bloodstains found at a criminal scene are often diluted and hemolyzed with water. Few papers dealing with the accuracy of the absorption-elution test in such stains have been published. In this study, a semiquantitative analysis of the absorption-elution test was performed using bloodstains made by dropping whole blood and blood dilute with various solvents onto filter papers, cotton, silk, nylon, polyester, polyestercotton, linen, wool, and rayon. When blood samples obtained from 30 different (group A: 10, group B: 10, group AB: 10) adults were diluted with normal saline or distilled water and dropped onto filter papers, detection limits of group A antigen for the absorption-elution test were 8-fold or 16-fold dilution with either solvent; those of group B antigen, 16-fold or 32-fold. When the same blood (group A, B, AB: leach) was diluted with normal saline or distilled water and dropped onto various materials, detection limits of group A antigen for the test were 4-fold or 8-fold dilution with either solvent for all 9 materials; those of group B, 16-fold or 32-fold dilution. When the same blood (group A, B, AB: leach) was diluted with various solvents and dropped onto filter papers, detection limits of group A antigen for the test were 4-fold or 8-fold dilution with all 9 solvents; those of group B, 16-fold or 32-fold dilution. The use of papain-treated red cells instead of non-treated cells improved the detection limit of group A antigen for the absorption-elution test into 32-fold dilution with normal saline and improved the detection limit of group B antigen into 64-fold dilution. When bloodstains were stored for up to 6 months under indirect illumination at room temperature, the detection limits of group A antigen for the test decreased to 2-fold dilution with the saline 6 months after storage; those of group B antigen, to 4-fold dilution.
Journal
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- Journal of The Showa Medical Association
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Journal of The Showa Medical Association 68 (3), 162-174, 2008
The Showa University Society
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Details 詳細情報について
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- CRID
- 1390282679810689280
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- NII Article ID
- 130001820491
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- NII Book ID
- AN00117027
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- ISSN
- 21850976
- 00374342
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- NDL BIB ID
- 9697890
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed