Energy-Dispersive X-Ray Microanalysis of the Artificial Caries-like Lesion of Human Enamel Embedded with and without Polyester Resin

  • KODAKA Tetsuo
    Department of Oral Histology, Showa University School of Dentistry
  • SANO Tsuneyoshi
    Department of Oral Anatomy, Showa University School of Dentistry

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説明

The mineralization values in the body of the artificially-demineralized caries-like lesion of human enamel, embedded with and without polyester resin, were analyzed on the transverse polished surfaces of 5 premolar teeth by energy-dispersive X-ray microanalysis (EDX) in SEM. In addition, the caries-like lesions were observed by backscattered electron (BSE) imaging and analyzed by wavelength-dispersive X-ray microanalysis (WDX). The resin-free samples had the caries-like body showing high BSE signals when compared with the rein-embedded samples. The high BSE-signal image will be caused by the ridges of the relatively-remineralized prism peripheries and the pits of the markedly-demineralized prism cores under mechanical grinding. The WDX line-profile of Ca-Kα in the resin-free samples was markedly decreased in the caries-like body when compared with that of the resin-embedded samples. The lower Ca content may be caused by the hollow of the caries-like body under mechanical grinding. In SEM-EDX, the caries-like body of the resin-free samples showed higher Ca values and tended to show higher P values than that of the resin-embedded samples. Such EDX data of the resin-free samples will be caused by porous structures in the caries-like body. In conclusion, when analyzed the natural caries and artificial caries-like lesions of enamel by SEM-EDX and WDX and also observed them by BSE, the samples should be embedded in infiltration resin in order to fill the porous structures and to form the smooth polished plane.

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