HISTOCHEMICAL STUDY OF SULFHYDRYL GROUP IN PILOMATRIXOMA, KERATOSE AND KERATOTIC TUMORS BY DACM STAINING METHOD

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  • ONO Mariko
    Department of Dermatology, School of Medicine, Showa University
  • KAWAGUCHI Noriko
    Department of Dermatology, School of Medicine, Showa University

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Other Title
  • Pilomatrixomaおよび若干の角化症ならびに角化性腫瘍におけるDACM染色による-SH基, S-S結合の分布, 挙動について (第一報)
  • Pilomatrixomaおよび若干の角化症ならびに角化性腫瘍におけるDACM染色による-SH基,S-S結合の分布,挙動について-1-
  • Pilomatrixoma オヨビ ジャッカン ノ カッカショウ ナラビニ カ

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The distribution of sulffhydryl (SH) and disulfide (S-S) groups in pilomatrixoma and several diseases with abnormal keratinization was observed by the new histochemical staining method using n- (7-dimethylamino-4-methylcoumarinyl) -maleimide (DACM) after Ogawa's method. The results obtained were as follows : 1. Pilamotrixoma : The distribution of SH groups was great in transitional cells as the other authors' observation. 2. Trichlemmoma : SH groups were observed in basaloid cells rather than in clear cells. 3. Pilar cyst and epidermal cyst : The distribution of SH groups was the same as normal epidermis. However, in pilar cyst, there was no keratogeous zone which was rich in SH groups. 4. Squamous cell carcinoma and Keratoacanthoma : SH groups were abundant in cytoplasma and nuclei of the tumor cells. A brilliant fluorescence of S-S groups was revealed in individual cell keratinization and horn pearls. 5. Hyperkeratosis lenticularis perstans : It was suspected that SH rich protein was overproducted in granular layer. 6. Darier's disease : SH groups were observed not only in cell membrane of horny and granular layer, but also in cytoplasma of horny layer and acantholytic cells. 7. Psoriasis vulgaris : The distribution of SH groups was great in granular and parakeratotic horny layer befor treament. These results suggested that the new histochemical staining method using DACM had the advantage over previous techniques and much more usefull for the studies of the differentiation and keratinization of epidermis.

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