HISTOLOGICAL EVALUATION OF NEW FOOD ALLERGY MODEL IN REPEATED OVALBUMIN-SENSITIZED MICE

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  • OTO Hideyasu
    Department of Microbilogy, and Immunology, Showa University School of Medicine
  • SHIMAMURA Tadakatsu
    Department of Microbilogy, and Immunology, Showa University School of Medicine
  • NAKAMURA Toshinori
    Department of Pediatrics, Showa University School of Medicine
  • FUJITANI Sinobu
    Department of Pediatrics, Showa University School of Medicine
  • SAKAI Naho
    Department of Pediatrics, Showa University School of Medicine
  • KAMIYA Taro
    Department of Pediatrics, Showa University School of Medicine
  • UENO Kouzo
    Department of Pediatrics, Showa University School of Medicine
  • KITABAYASHI Taeru
    Department of Pediatrics, Showa University School of Medicine
  • ITABASHI Kazuo
    Department of Pediatrics, Showa University School of Medicine

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Other Title
  • 食物抗原の頻回投与による食物アレルギー慢性モデルマウス作製に関する検討
  • ショクモツ コウゲン ノ ヒンカイ トウヨ ニ ヨル ショクモツ アレルギー マンセイ モデル マウス サクセイ ニ カンスル ケントウ

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The number of pediatric patients with food allergies has recently been on the rise. One reason for the increase is the fact that the pathogenetic mechanisms of allergies are still poorly understood since any animal models to elucidate the pathology have not been established. In this study, therefore, we administered multiple doses of food allergens to mice in an attempt to establish a mouse model for food allergy in addition, we compared the new model with an existing model with respect to histopathological changes in the pathology. We used food allergy model mice sensitized with ovalbumin (OVA) to histopathologically examine the small intestine of mice in the control group, in the 5-week OVA short-term administration group, and in the 11-week OVA long-term administration group. The assessment items were as follows: length of villi; number of intraepithelial lymphocytes (IELs), goblet cells; and number of cryptopatches (CPs) . IELs were significantly (p < 0.05) more numerous in the OVA short-term administration group than in the control group. However, no significant difference was found in IELs between the OVA long-term administration group and the control group. The number of goblet cells was significantly (p < 0.05) greater in the OVA long-term administration group than in the control group, while no significant difference was found in the number between the OVA short-term administration group and the control group. The increased number of goblet cells in the OVA long-term administration group was conjectured attributable to the mechanism of repair and defense triggered by repeated immune responses due to sensitization with OVA. We consider that further research on the relevance of long-term exposure-elicited food allergy experimental model, along with the evaluation of immune response, is required.

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