Confirmation of Existence of Three Types of Mesenchymal Progenitor Cells in Cultured Human Periodontal Ligament Cells
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- OKUTSU Tomohiko
- Department of Periodontology, Showa University School of Dentistry
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- KOBAYASHI Makoto
- Department of Periodontology, Showa University School of Dentistry
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- KOIDE Yoko
- Department of Periodontology, Showa University School of Dentistry
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- TAKADA Takatora
- Department of Periodontology, Showa University School of Dentistry
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- TAKIGUCHI Takashi
- Department of Periodontology, Showa University School of Dentistry
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- YAMAMOTO Matsuo
- Department of Periodontology, Showa University School of Dentistry
Bibliographic Information
- Other Title
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- 培養歯根膜細胞中における各種問葉系前駆細胞の存在確認
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Abstract
The transplantation of ex vivo-expanded human periodontal ligament (HPL) cells or mesenchymal stem cell (MSC) -rich population isolated from the HPL cells with scaffolds into periodontal defects appears to be a powerful strategy to promote periodontal regeneration. However, up to date, there have been few direct evidences that each precursor of various mesenchymal cell types and MSC exist in the heterogeneous HPL cell cultures. In this study, we qualitatively estimated a potential to differentiate into three types of mesenchymal cells in the HPL cells, as compared with that in human bone marrow-derived MSC (HMSC), which were used as a control. As results, the HPL cells contained many osteoblast lineage cells and small number of adipocyte precursors, while chondrocyte precursors might exist at slight levels or not in the cells. Further, alkaline phosphatase (ALP) negative cell-rich (ALPLow) fraction isolated from the HPL cells contained relatively more adipocyte precursors.
Journal
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- The Journal of Showa University Dental Society
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The Journal of Showa University Dental Society 27 (3), 165-172, 2007
Showa University Dental Society
