IMMUNOGENIC POTENTIALITY OF BACTERIAL LIPID A COMPONENTS IN CARRIER-FREE FORMS TO MOUSE SPLEEN CELLS
-
- Mita Akira
- Department of Microbial Chemistry, Showa University School of Pharmaceutical Sciences
-
- Ohta Hidekazu
- Department of 2nd Pathology, Showa University School of Medicine
Bibliographic Information
- Other Title
-
- マウス脾細胞に対するCarrier-Freeのバクテリア由来lipid A成分の免疫原性
- マウス脾細胞に対するCarrier-Freeのバクテリヤ由来lipid A成分の免疫原性〔英文〕
- マウス ヒ サイボウ ニ タイスル Carrier Free ノ バクテリヤ
Search this article
Abstract
Immunogenic activities of an alkali-treated lipid A and a heptoseless glycolipid (KDO-lipid A) derived from a R595 rough mutant of Salmonella minnesota were investigated in male mice by the methods of Biozzi et al. and Cunningham.<BR>Treatments with suitable forms of the materials led to occurrence of plaque- and rosette-forming cells with specific antibodies for alkali-treated lipid A coated to sheep erythrocytes. Following two subcutaneous treatments with Freund's adjuvants, intravenous injection of a complex of alkali-treated lipid A with acid-heated bacterial cells activated the mouse spleen cells to contain direct plaque-forming cells with anti-lipid A antibodies. Moreover, two intravenous sensitizations of the complex could induce syntheses of IgM and IgG antibodies with the specificity. Even when alkali-treated lipid A or glycolipid in a carrier-free form was intravenously introduced, mice were able to produce the anti-lipid A antibody. On intraperitoneal immunization with the lipid A alone, mice had no plaque-forming cells, but rosette-forming cells in the spleen. However, mice receiving the same treatment with the glycolipid alone had two types of the antibody-producing cells simultaneously.<BR>The anti-lipid A antibody-producing cells were also observed in thymus cells obtained from glycolipid-treated group.
Journal
-
- Journal of The Showa Medical Association
-
Journal of The Showa Medical Association 38 (3), 263-272, 1978
The Showa University Society
- Tweet
Details 詳細情報について
-
- CRID
- 1390282679815468160
-
- NII Article ID
- 130001823214
-
- NII Book ID
- AN00117027
-
- ISSN
- 21850976
- 00374342
-
- NDL BIB ID
- 2073133
-
- Data Source
-
- JaLC
- NDL
- CiNii Articles
-
- Abstract License Flag
- Disallowed