ON CONSUMPTION OF LACTOSE AND SLIME PRODUCTION BY MUCOID M DERIVED FROM M-TYPE VAR. MURASE

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  • KITANI Fusako
    Department of Bacteriology, School of Medicine, Showa University

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  • M-type Var. Muraseより変異した粘液型MのLactose消費とSlime合成
  • M-type var.murase ヨリ ヘンイ シタ ネンエキガタ M ノ lactose ショウヒ ト slime ゴウセイ

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Abstract

Mucoid M of Salm. typhi-murium produces slime by taking up free galactose from the medium, but no slime is produced when lactose is used. While the one of E. coli produces slime both from galactose and lactose. A study is directed to clarify this problem. The results are as follows.<BR>1) It was possible to determine galactose in a dose over 2γ/0.1 ml and lactose over 16γ/0.1 ml by the cup-plate method using M of Murase.<BR>2) More amount of lactose was utilized by mucoid M than M when cultured on the lactose agar plate.<BR>3) Extract of the medium of lactose plate culture of M or mucoid M of E. coli revealed only galactose spot by paperchromatography.<BR>4) When variants of E. coli were cultured onto the outer circle of the lactose agar plate and the mucoid M of Salm, typhi-murium was inoculated onto the inner area of the plate, the production of free galactose was recognized in the mucoid growth of the latter, whereas no mucoid colony was formed when the original galactose splitting strain of E. coli was used, probably because of the further decomposition of galactose.<BR>5) When M, mocoid M or MT (galactose negative and galactose resistant variant of M) was cultured onlo the outer side of lactose agar plate one night, and then the agar discs were punched up by the corkborer from the inner area of the plate and placed on the seed layer of M, one night, the production of galactose was proved by formation of clear inhibi-tion zone around the disc as the control.<BR>6) Extract of the same agar disc showed the spots coresponding to lactose and galactose or glucose on paperchromatography.<BR>7) Extracts from the lactose agar plates on which M, mucoid M or MT of E. coli was cultured showed the galactose spot only on paperchromatography, when it had been treated with MT in advance.<BR>Thus, it is believed that first, lactose is divided into two parts, galactose and glucose, by the β-galactosidase of E. coli and secondarily, some amount of this galactose moiety is taken up both by M and mucoid M, but more amount is taken up by mucoid M, for the production of slime.

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