径８ｍｍの大腸ｓｓ癌の１例とその細胞増殖能について　Ｋｉ‐６７／ＡｇＮＯＲ二重染色を用いて

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  • A Case of Advanced Colon Cancer Measuring 8 mm in Diameter.
  • Ki-67/AgNOR二重染色を用いて

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A 68 year-old male who had underwent sigmoidectomy for colon cancer on April 6, 1992 was admitted to our hospital. Colonoscopy revealed a small flat-elevated lesion with converging folds of the ascending colon. On preoperative evaluation of a massive invasion to the submucosal layer, right hemicolectomy was perforemed on September 8, 1994. Macroscopic examination of the resected specimen showed a slightly elevated tumor with converging folds, measuring 5 x 8 mm in diameter. Histopathology revealed a well differentiated adenocarcinoma invading to the subserosal layer. No recidual adenomatous tissue was identified, and no metastasis was found in 14 lymph nodes.<BR>The proliferating activity of this small advanced colon cancer was determined by double staining technique of Ki - 67 and argyrophilic nucleolar organizer resions (Ag-NOR) using formalin-fixed paraffin-embedded sections. Labeling index of Ki-67 monoclonal antibody (MIB 1) was 7.3 %. Although the Ki-67 negative cells had low AgNOR counts (mean 2.50), the Ki-67 positive cells had high AgNOR counts (mean 9.98 per nucleus).

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