妊娠中毒症の尿中TMに関する研究
書誌事項
- タイトル別名
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- Studies on urinary thrombomodulin in toxemia of pregnancy.
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説明
Thrombomodulin (TM) is known to exist in the glomerulus and to be excreted in urine. To elucidate biochemical properties of urinary TM in normal and toxemic pregnancy, the levels of immunoreactive TM (TM antigen) and its activities were measured by ELISA and by a protein C activation method, respectively. The samples for measurements were prapered from urine by DIP-thrombin agarose affinity chromatography.<br>The urinary protein fraction bound to DIP-thrombin agarose, obtained from normal pregnants, pregnants with early onsets type toxemia (24-28 weeks of gestation), and with late onset type toxemia (later than 32 weeks) commonly showed a single band with a mol. wt. 63Kd in SDS-polyacrylamide gel electrophoresis with silver staining.<br>The levels of urinary TM antigen and TM activity in normal pregnancy increased with gestation weeks. Those in late onset type pregnancy showed similar chnges. There was no difference in the ratio of TM activity/antigen between these two types of pregnancy. However, in early onset type toxemia the level of TM activity was low, and, further, the ratio of TM activity/antigen was extremely low.<br>The TM in early onset type toxemia had a significantly lower Vmax than that in normal pregnancy. These findings suggested that the TM in early onset type toxemia was functionally impaired as a cofactor for thrombin.
収録刊行物
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- 血液と脈管
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血液と脈管 20 (2), 197-200, 1989
一般社団法人 日本血栓止血学会
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詳細情報 詳細情報について
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- CRID
- 1390282679834797824
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- NII論文ID
- 130004261515
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- ISSN
- 18842372
- 03869717
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可